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goat anti olig2  (R&D Systems)


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    R&D Systems goat anti olig2
    (A) Schematic showing markers used to label and visualize oligodendrocyte progenitor cells (OPCs), premyelinating oligodendrocytes (preOLs), and myelinating oligodendrocytes (myelinating OLs). (B) Schematic showing the design of Enpp6-IRES-CreER T2 driver line. The IRES-CreER T2 cassette was inserted into the 3’UTR of Enpp6 , immediately after the stop codon in exon 8. (C) Sagittal brain section of postnatal day 9 (P9) Enpp6-CreER T2 ; Ai9/+ mouse 1 day post injection (dpi) with 150 mg/kg 4-hydroxytamoxifen (4HT). The DsRed + cells were visualized by immunohistochemistry using an anti-DsRed antibody. The red insets mark the regions selected for high-magnification images at corpus callosum (#1) and cerebellum (#2). (D) High magnification images of #1 and #2 insets in (C) showing the ramified, “spider-like” morphology distinctly observed in preOLs. (E) Representative images of P9 Enpp6-CreER T2 ; Ai9/+ cortex administered with 150 mg/kg 4HT at 1 dpi using antibodies raised against DsRed (red) and <t>Olig2</t> (green). (F) Quantifications of the proportion of DsRed + Olig2 + cells over the total number of DsRed + cells in P9 Enpp6-CreER T2 ; Ai9/+ cortices. (G and H) Immunohistochemistry (G) and quantification (H) of P9 Enpp6-CreER T2 ; Ai9/+ brain sections stained with DsRed (red) and Sox10 (green) antibodies. (I and I’) Representative confocal images of P9 Enpp6-CreER T2 ; Ai9/+ optic nerve (I) and spinal cord (I’) stained with DsRed (red) and Olig2 (green) antibodies. (J) Immunohistochemistry combined with RNAScope using DsRed antibody (red), Enpp6 probe (green), and LncOL1 probe (cyan) in P9 Enpp6-CreER T2 ; Ai9/+ cortex. (K and L) Quantifications of the proportion of Enpp6 + DsRed + cells (K) and LncOL1 + DsRed + cells (L) among the total DsRed + cells. (M) Quantification of Mog + DsRed + cell proportion among the total DsRed + cells. (N and O) Quantifications of the proportions of Enpp6 + DsRed + and LncOL1 + DsRed + cells among the total Enpp6 + cells (N) and LncOL1 + cells (O), respectively. Error bars represent SEM. Scale bars: 1 mm in (C); 50 µm in (D), (E), (G), (I), (I’), and (J). Close circles in (F), (H), and (K)-(O) represent individual animals. n=3 animals per category. CC: Corpus Callosum; Hippo: Hippocampus; Cblm: Cerebellum; Brnstm: Brainstem.
    Goat Anti Olig2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Genetically Labeled Premyelinating Oligodendrocytes: Bridging Oligodendrogenesis and Neuronal Activity"

    Article Title: Genetically Labeled Premyelinating Oligodendrocytes: Bridging Oligodendrogenesis and Neuronal Activity

    Journal: bioRxiv

    doi: 10.1101/2024.12.27.630559

    (A) Schematic showing markers used to label and visualize oligodendrocyte progenitor cells (OPCs), premyelinating oligodendrocytes (preOLs), and myelinating oligodendrocytes (myelinating OLs). (B) Schematic showing the design of Enpp6-IRES-CreER T2 driver line. The IRES-CreER T2 cassette was inserted into the 3’UTR of Enpp6 , immediately after the stop codon in exon 8. (C) Sagittal brain section of postnatal day 9 (P9) Enpp6-CreER T2 ; Ai9/+ mouse 1 day post injection (dpi) with 150 mg/kg 4-hydroxytamoxifen (4HT). The DsRed + cells were visualized by immunohistochemistry using an anti-DsRed antibody. The red insets mark the regions selected for high-magnification images at corpus callosum (#1) and cerebellum (#2). (D) High magnification images of #1 and #2 insets in (C) showing the ramified, “spider-like” morphology distinctly observed in preOLs. (E) Representative images of P9 Enpp6-CreER T2 ; Ai9/+ cortex administered with 150 mg/kg 4HT at 1 dpi using antibodies raised against DsRed (red) and Olig2 (green). (F) Quantifications of the proportion of DsRed + Olig2 + cells over the total number of DsRed + cells in P9 Enpp6-CreER T2 ; Ai9/+ cortices. (G and H) Immunohistochemistry (G) and quantification (H) of P9 Enpp6-CreER T2 ; Ai9/+ brain sections stained with DsRed (red) and Sox10 (green) antibodies. (I and I’) Representative confocal images of P9 Enpp6-CreER T2 ; Ai9/+ optic nerve (I) and spinal cord (I’) stained with DsRed (red) and Olig2 (green) antibodies. (J) Immunohistochemistry combined with RNAScope using DsRed antibody (red), Enpp6 probe (green), and LncOL1 probe (cyan) in P9 Enpp6-CreER T2 ; Ai9/+ cortex. (K and L) Quantifications of the proportion of Enpp6 + DsRed + cells (K) and LncOL1 + DsRed + cells (L) among the total DsRed + cells. (M) Quantification of Mog + DsRed + cell proportion among the total DsRed + cells. (N and O) Quantifications of the proportions of Enpp6 + DsRed + and LncOL1 + DsRed + cells among the total Enpp6 + cells (N) and LncOL1 + cells (O), respectively. Error bars represent SEM. Scale bars: 1 mm in (C); 50 µm in (D), (E), (G), (I), (I’), and (J). Close circles in (F), (H), and (K)-(O) represent individual animals. n=3 animals per category. CC: Corpus Callosum; Hippo: Hippocampus; Cblm: Cerebellum; Brnstm: Brainstem.
    Figure Legend Snippet: (A) Schematic showing markers used to label and visualize oligodendrocyte progenitor cells (OPCs), premyelinating oligodendrocytes (preOLs), and myelinating oligodendrocytes (myelinating OLs). (B) Schematic showing the design of Enpp6-IRES-CreER T2 driver line. The IRES-CreER T2 cassette was inserted into the 3’UTR of Enpp6 , immediately after the stop codon in exon 8. (C) Sagittal brain section of postnatal day 9 (P9) Enpp6-CreER T2 ; Ai9/+ mouse 1 day post injection (dpi) with 150 mg/kg 4-hydroxytamoxifen (4HT). The DsRed + cells were visualized by immunohistochemistry using an anti-DsRed antibody. The red insets mark the regions selected for high-magnification images at corpus callosum (#1) and cerebellum (#2). (D) High magnification images of #1 and #2 insets in (C) showing the ramified, “spider-like” morphology distinctly observed in preOLs. (E) Representative images of P9 Enpp6-CreER T2 ; Ai9/+ cortex administered with 150 mg/kg 4HT at 1 dpi using antibodies raised against DsRed (red) and Olig2 (green). (F) Quantifications of the proportion of DsRed + Olig2 + cells over the total number of DsRed + cells in P9 Enpp6-CreER T2 ; Ai9/+ cortices. (G and H) Immunohistochemistry (G) and quantification (H) of P9 Enpp6-CreER T2 ; Ai9/+ brain sections stained with DsRed (red) and Sox10 (green) antibodies. (I and I’) Representative confocal images of P9 Enpp6-CreER T2 ; Ai9/+ optic nerve (I) and spinal cord (I’) stained with DsRed (red) and Olig2 (green) antibodies. (J) Immunohistochemistry combined with RNAScope using DsRed antibody (red), Enpp6 probe (green), and LncOL1 probe (cyan) in P9 Enpp6-CreER T2 ; Ai9/+ cortex. (K and L) Quantifications of the proportion of Enpp6 + DsRed + cells (K) and LncOL1 + DsRed + cells (L) among the total DsRed + cells. (M) Quantification of Mog + DsRed + cell proportion among the total DsRed + cells. (N and O) Quantifications of the proportions of Enpp6 + DsRed + and LncOL1 + DsRed + cells among the total Enpp6 + cells (N) and LncOL1 + cells (O), respectively. Error bars represent SEM. Scale bars: 1 mm in (C); 50 µm in (D), (E), (G), (I), (I’), and (J). Close circles in (F), (H), and (K)-(O) represent individual animals. n=3 animals per category. CC: Corpus Callosum; Hippo: Hippocampus; Cblm: Cerebellum; Brnstm: Brainstem.

    Techniques Used: Injection, Immunohistochemistry, Staining, RNAscope



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    (A) Schematic showing markers used to label and visualize oligodendrocyte progenitor cells (OPCs), premyelinating oligodendrocytes (preOLs), and myelinating oligodendrocytes (myelinating OLs). (B) Schematic showing the design of Enpp6-IRES-CreER T2 driver line. The IRES-CreER T2 cassette was inserted into the 3’UTR of Enpp6 , immediately after the stop codon in exon 8. (C) Sagittal brain section of postnatal day 9 (P9) Enpp6-CreER T2 ; Ai9/+ mouse 1 day post injection (dpi) with 150 mg/kg 4-hydroxytamoxifen (4HT). The DsRed + cells were visualized by immunohistochemistry using an anti-DsRed antibody. The red insets mark the regions selected for high-magnification images at corpus callosum (#1) and cerebellum (#2). (D) High magnification images of #1 and #2 insets in (C) showing the ramified, “spider-like” morphology distinctly observed in preOLs. (E) Representative images of P9 Enpp6-CreER T2 ; Ai9/+ cortex administered with 150 mg/kg 4HT at 1 dpi using antibodies raised against DsRed (red) and <t>Olig2</t> (green). (F) Quantifications of the proportion of DsRed + Olig2 + cells over the total number of DsRed + cells in P9 Enpp6-CreER T2 ; Ai9/+ cortices. (G and H) Immunohistochemistry (G) and quantification (H) of P9 Enpp6-CreER T2 ; Ai9/+ brain sections stained with DsRed (red) and Sox10 (green) antibodies. (I and I’) Representative confocal images of P9 Enpp6-CreER T2 ; Ai9/+ optic nerve (I) and spinal cord (I’) stained with DsRed (red) and Olig2 (green) antibodies. (J) Immunohistochemistry combined with RNAScope using DsRed antibody (red), Enpp6 probe (green), and LncOL1 probe (cyan) in P9 Enpp6-CreER T2 ; Ai9/+ cortex. (K and L) Quantifications of the proportion of Enpp6 + DsRed + cells (K) and LncOL1 + DsRed + cells (L) among the total DsRed + cells. (M) Quantification of Mog + DsRed + cell proportion among the total DsRed + cells. (N and O) Quantifications of the proportions of Enpp6 + DsRed + and LncOL1 + DsRed + cells among the total Enpp6 + cells (N) and LncOL1 + cells (O), respectively. Error bars represent SEM. Scale bars: 1 mm in (C); 50 µm in (D), (E), (G), (I), (I’), and (J). Close circles in (F), (H), and (K)-(O) represent individual animals. n=3 animals per category. CC: Corpus Callosum; Hippo: Hippocampus; Cblm: Cerebellum; Brnstm: Brainstem.
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    (A) Schematic showing markers used to label and visualize oligodendrocyte progenitor cells (OPCs), premyelinating oligodendrocytes (preOLs), and myelinating oligodendrocytes (myelinating OLs). (B) Schematic showing the design of Enpp6-IRES-CreER T2 driver line. The IRES-CreER T2 cassette was inserted into the 3’UTR of Enpp6 , immediately after the stop codon in exon 8. (C) Sagittal brain section of postnatal day 9 (P9) Enpp6-CreER T2 ; Ai9/+ mouse 1 day post injection (dpi) with 150 mg/kg 4-hydroxytamoxifen (4HT). The DsRed + cells were visualized by immunohistochemistry using an anti-DsRed antibody. The red insets mark the regions selected for high-magnification images at corpus callosum (#1) and cerebellum (#2). (D) High magnification images of #1 and #2 insets in (C) showing the ramified, “spider-like” morphology distinctly observed in preOLs. (E) Representative images of P9 Enpp6-CreER T2 ; Ai9/+ cortex administered with 150 mg/kg 4HT at 1 dpi using antibodies raised against DsRed (red) and Olig2 (green). (F) Quantifications of the proportion of DsRed + Olig2 + cells over the total number of DsRed + cells in P9 Enpp6-CreER T2 ; Ai9/+ cortices. (G and H) Immunohistochemistry (G) and quantification (H) of P9 Enpp6-CreER T2 ; Ai9/+ brain sections stained with DsRed (red) and Sox10 (green) antibodies. (I and I’) Representative confocal images of P9 Enpp6-CreER T2 ; Ai9/+ optic nerve (I) and spinal cord (I’) stained with DsRed (red) and Olig2 (green) antibodies. (J) Immunohistochemistry combined with RNAScope using DsRed antibody (red), Enpp6 probe (green), and LncOL1 probe (cyan) in P9 Enpp6-CreER T2 ; Ai9/+ cortex. (K and L) Quantifications of the proportion of Enpp6 + DsRed + cells (K) and LncOL1 + DsRed + cells (L) among the total DsRed + cells. (M) Quantification of Mog + DsRed + cell proportion among the total DsRed + cells. (N and O) Quantifications of the proportions of Enpp6 + DsRed + and LncOL1 + DsRed + cells among the total Enpp6 + cells (N) and LncOL1 + cells (O), respectively. Error bars represent SEM. Scale bars: 1 mm in (C); 50 µm in (D), (E), (G), (I), (I’), and (J). Close circles in (F), (H), and (K)-(O) represent individual animals. n=3 animals per category. CC: Corpus Callosum; Hippo: Hippocampus; Cblm: Cerebellum; Brnstm: Brainstem.

    Journal: bioRxiv

    Article Title: Genetically Labeled Premyelinating Oligodendrocytes: Bridging Oligodendrogenesis and Neuronal Activity

    doi: 10.1101/2024.12.27.630559

    Figure Lengend Snippet: (A) Schematic showing markers used to label and visualize oligodendrocyte progenitor cells (OPCs), premyelinating oligodendrocytes (preOLs), and myelinating oligodendrocytes (myelinating OLs). (B) Schematic showing the design of Enpp6-IRES-CreER T2 driver line. The IRES-CreER T2 cassette was inserted into the 3’UTR of Enpp6 , immediately after the stop codon in exon 8. (C) Sagittal brain section of postnatal day 9 (P9) Enpp6-CreER T2 ; Ai9/+ mouse 1 day post injection (dpi) with 150 mg/kg 4-hydroxytamoxifen (4HT). The DsRed + cells were visualized by immunohistochemistry using an anti-DsRed antibody. The red insets mark the regions selected for high-magnification images at corpus callosum (#1) and cerebellum (#2). (D) High magnification images of #1 and #2 insets in (C) showing the ramified, “spider-like” morphology distinctly observed in preOLs. (E) Representative images of P9 Enpp6-CreER T2 ; Ai9/+ cortex administered with 150 mg/kg 4HT at 1 dpi using antibodies raised against DsRed (red) and Olig2 (green). (F) Quantifications of the proportion of DsRed + Olig2 + cells over the total number of DsRed + cells in P9 Enpp6-CreER T2 ; Ai9/+ cortices. (G and H) Immunohistochemistry (G) and quantification (H) of P9 Enpp6-CreER T2 ; Ai9/+ brain sections stained with DsRed (red) and Sox10 (green) antibodies. (I and I’) Representative confocal images of P9 Enpp6-CreER T2 ; Ai9/+ optic nerve (I) and spinal cord (I’) stained with DsRed (red) and Olig2 (green) antibodies. (J) Immunohistochemistry combined with RNAScope using DsRed antibody (red), Enpp6 probe (green), and LncOL1 probe (cyan) in P9 Enpp6-CreER T2 ; Ai9/+ cortex. (K and L) Quantifications of the proportion of Enpp6 + DsRed + cells (K) and LncOL1 + DsRed + cells (L) among the total DsRed + cells. (M) Quantification of Mog + DsRed + cell proportion among the total DsRed + cells. (N and O) Quantifications of the proportions of Enpp6 + DsRed + and LncOL1 + DsRed + cells among the total Enpp6 + cells (N) and LncOL1 + cells (O), respectively. Error bars represent SEM. Scale bars: 1 mm in (C); 50 µm in (D), (E), (G), (I), (I’), and (J). Close circles in (F), (H), and (K)-(O) represent individual animals. n=3 animals per category. CC: Corpus Callosum; Hippo: Hippocampus; Cblm: Cerebellum; Brnstm: Brainstem.

    Article Snippet: Primary antibodies used in this study include: Rabbit Anti-DsRed (Clonetech, 1:1000, Cat#632496), Goat Anti-DsRed (Biorybt, 1:1000, Cat#orb11618), Goat Anti-Olig2 (R&D systems, 1:1000, Cat#AF2418SP), Goat Anti-Sox10 (R&D systems, 1:1000, Cat#AF2864), Mouse Anti-CC1 (Millipore, 1:200, Cat#OP80), Rat Anti-MBP (abcam, 1:4000, Cat#7349), Rabbit Anti-NeuN (Cell Signaling, 1:1000, Cat#12943), Chicken Anti-GFAP (Aves Lab, 1:2000, Cat#GFAP, AB_2313547), Rabbit Anti-Iba1 (Wako Chemicals, 1:1000, Cat#019-19741), Rabbit Anti-BCAS1 (Synaptic Systems, 1:1000, Cat#445003) and Rabbit Anti-PDGFRα (abcam, 1:1000, Cat#ab203491).

    Techniques: Injection, Immunohistochemistry, Staining, RNAscope